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BioMed Research International
Volume 2013 (2013), Article ID 878930, 10 pages
http://dx.doi.org/10.1155/2013/878930
Research Article

Evaluation of a Thiolated Chitosan Scaffold for Local Delivery of BMP-2 for Osteogenic Differentiation and Ectopic Bone Formation

1Department of Pharmacology and Dental Therapeutics, School of Dentistry, Chonnam National University, Gwangju 500-757, Republic of Korea
2Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, Gwangju 500-757, Republic of Korea
3Dental Science Research Institute, School of Dentistry, Chonnam National University, Gwangju, 500-757, Republic of Korea

Received 4 April 2013; Revised 12 July 2013; Accepted 15 July 2013

Academic Editor: Joshua R. Mauney

Copyright © 2013 In-Ho Bae et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. T. W. Bauer and G. F. Muschler, “Bone graft materials: an overview of the basic science,” Clinical Orthopaedics and Related Research, no. 371, pp. 10–27, 2000. View at Google Scholar · View at Scopus
  2. A. J. Celeste, J. A. Iannazzi, R. C. Taylor et al., “Identification of transforming growth factor β family members present in bone-inductive protein purified from bovine bone,” Proceedings of the National Academy of Sciences of the United States of America, vol. 87, no. 24, pp. 9843–9847, 1990. View at Publisher · View at Google Scholar · View at Scopus
  3. E. A. Wang, “Bone morphogenetic proteins (BMPs): therapeutic potential in healing bony defects,” Trends in Biotechnology, vol. 11, no. 9, pp. 379–383, 1993. View at Publisher · View at Google Scholar · View at Scopus
  4. E. Ozkaynak, D. C. Rueger, E. A. Drier et al., “OP-1 cDNA encodes an osteogenic protein in the TGF-β family,” EMBO Journal, vol. 9, no. 7, pp. 2085–2093, 1990. View at Google Scholar · View at Scopus
  5. T. K. Sampath, J. E. Coughlin, R. M. Whetstone et al., “Bovine osteogenic protein is composed of dimers of OP-1 and BMP-2A, two members of the transforming growth factor-β superfamily,” Journal of Biological Chemistry, vol. 265, no. 22, pp. 13198–13205, 1990. View at Google Scholar · View at Scopus
  6. E. Ozkaynak, P. N. J. Schnegelsberg, D. F. Jin et al., “Osteogenic protein-2. A new member of the transforming growth factor-β superfamily expressed early in embryogenesis,” Journal of Biological Chemistry, vol. 267, no. 35, pp. 25220–25227, 1992. View at Google Scholar · View at Scopus
  7. K. Elima, “Osteoinductive proteins,” Annals of Medicine, vol. 25, no. 4, pp. 395–402, 1993. View at Google Scholar · View at Scopus
  8. A. H. Reddi and N. S. Cunningham, “Initiation and promotion of bone differentiation by bone morphogenetic proteins,” Journal of Bone and Mineral Research, vol. 8, no. 2, pp. S499–S502, 1993. View at Google Scholar · View at Scopus
  9. I. Ono, T. Ohura, M. Murata, H. Yamaguchi, Y. Ohnuma, and Y. Kuboki, “A study on bone induction in hydroxyapatite combined with bone morphogenetic protein,” Plastic and Reconstructive Surgery, vol. 90, no. 5, pp. 870–879, 1992. View at Google Scholar · View at Scopus
  10. A. W. Yasko, J. M. Lane, E. J. Fellinger, V. Rosen, J. M. Wozney, and E. A. Wang, “The healing of segmental bone defects, induced by recombinant human bone morphogenetic protein (rhBMP-2). A radiographic, histological, and biomechanical study in rats,” Journal of Bone and Joint Surgery, vol. 74, no. 5, pp. 659–670, 1992. View at Google Scholar · View at Scopus
  11. H. D. Zegzula, D. C. Buck, J. Brekke, J. M. Wozney, and J. O. Hollinger, “Bone formation with use of rhBMP-2 (recombinant human bone morphogenetic protein-2),” Journal of Bone and Joint Surgery, vol. 79, no. 12, pp. 1778–1790, 1997. View at Google Scholar · View at Scopus
  12. R. Kenley, L. Marden, T. Turek, L. Jin, E. Ron, and J. O. Hollinger, “Osseous regeneration in the rat calvarium using novel delivery systems for recombinant human bone morphogenetic protein-2 (rhBMP-2),” Journal of Biomedical Materials Research, vol. 28, no. 10, pp. 1139–1147, 1994. View at Publisher · View at Google Scholar · View at Scopus
  13. Z. Schwartz, A. Somers, J. T. Mellonig et al., “Addition of human recombinant bone morphogenetic protein-2 to inactive commercial human demineralized freeze-dried bone allograft makes an effective composite bone inductive implant material,” Journal of Periodontology, vol. 69, no. 12, pp. 1337–1345, 1998. View at Google Scholar · View at Scopus
  14. G. Haipeng, Z. Yinghui, L. Jianchun et al., “Studies on nerve cell affinity of chitosan-derived materials,” Journal of Biomedical Materials Research A, vol. 52, no. 2, pp. 285–295, 2000. View at Google Scholar
  15. J. L. Drury and D. J. Mooney, “Hydrogels for tissue engineering: scaffold design variables and applications,” Biomaterials, vol. 24, no. 24, pp. 4337–4351, 2003. View at Publisher · View at Google Scholar · View at Scopus
  16. S. N. Kulikov, I. A. Tiurin, R. S. Fassakhov, and V. P. Varlamov, “Antibacterial and antimycotic activity of chitosan: mechanisms of action and role of the structure,” Zhurnal mikrobiologii, Epidemiologii, i Immunobiologii, no. 5, pp. 91–97, 2009. View at Google Scholar · View at Scopus
  17. Y. Furusho, A. Sabarudin, L. Hakim, K. Oshita, M. Oshima, and S. Motomizu, “Automated pretreatment system for the speciation of Cr(III) and Cr(VI) using dual mini-columns packed with newly synthesized chitosan resin and ME-03 resin,” Analytical Sciences, vol. 25, no. 1, pp. 51–56, 2009. View at Publisher · View at Google Scholar · View at Scopus
  18. L. Richert, P. Lavalle, E. Payan et al., “Layer by layer buildup of polysaccharide films: physical chemistry and cellular adhesion aspects,” Langmuir, vol. 20, no. 2, pp. 448–458, 2004. View at Publisher · View at Google Scholar · View at Scopus
  19. L. Illum, “Chitosan and its use as a pharmaceutical excipient,” Pharmaceutical Research, vol. 15, no. 9, pp. 1326–1331, 1998. View at Publisher · View at Google Scholar · View at Scopus
  20. A. Lahiji, A. Sohrabi, D. S. Hungerford, and C. G. Frondoza, “Chitosan supports the expression of extracellular matrix proteins in human osteoblasts and chondrocytes,” Journal of Biomedical Materials Research A, vol. 51, no. 4, pp. 586–595, 2000. View at Google Scholar
  21. A. Montembault, C. Viton, and A. Domard, “Physico-chemical studies of the gelation of chitosan in a hydroalcoholic medium,” Biomaterials, vol. 26, no. 8, pp. 933–943, 2005. View at Publisher · View at Google Scholar · View at Scopus
  22. F. Cui, F. Qian, Z. Zhao, L. Yin, C. Tang, and C. Yin, “Preparation, characterization, and oral delivery of insulin loaded carboxylated chitosan grafted poly(methyl methacrylate) nanoparticles,” Biomacromolecules, vol. 10, no. 5, pp. 1253–1258, 2009. View at Publisher · View at Google Scholar · View at Scopus
  23. Y.-C. Lin, F.-J. Tan, K. G. Marra, S.-S. Jan, and D.-C. Liu, “Synthesis and characterization of collagen/hyaluronan/chitosan composite sponges for potential biomedical applications,” Acta Biomaterialia, vol. 5, no. 7, pp. 2591–2600, 2009. View at Publisher · View at Google Scholar · View at Scopus
  24. A. Bernkop-Schnürch, M. Hornof, and D. Guggi, “Thiolated chitosans,” European Journal of Pharmaceutics and Biopharmaceutics, vol. 57, no. 1, pp. 9–17, 2004. View at Publisher · View at Google Scholar · View at Scopus
  25. M.-S. Kim, Y.-J. Choi, I. Noh, and G. Tae, “Synthesis and characterization of in situ chitosan-based hydrogel via grafting of carboxyethyl acrylate,” Journal of Biomedical Materials Research A, vol. 83, no. 3, pp. 674–682, 2007. View at Publisher · View at Google Scholar · View at Scopus
  26. A. Bernkop-Schnürch, M. Hornof, and T. Zoidl, “Thiolated polymers—thiomers: synthesis and in vitro evaluation of chitosan-2-iminothiolane conjugates,” International Journal of Pharmaceutics, vol. 260, no. 2, pp. 229–237, 2003. View at Publisher · View at Google Scholar · View at Scopus
  27. C. E. Kast, C. Valenta, M. Leopold, and A. Bernkop-Schnürch, “Design and in vitro evaluation of a novel bioadhesive vaginal drug delivery system for clotrimazole,” Journal of Controlled Release, vol. 81, no. 3, pp. 347–354, 2002. View at Publisher · View at Google Scholar · View at Scopus
  28. D. Guggi, A. H. Krauland, and A. Bernkop-Schnürch, “Systemic peptide delivery via the stomach: in vivo evaluation of an oral dosage form for salmon calcitonin,” Journal of Controlled Release, vol. 92, no. 1-2, pp. 125–135, 2003. View at Publisher · View at Google Scholar · View at Scopus
  29. L. Yin, J. Ding, C. He, L. Cui, C. Tang, and C. Yin, “Drug permeability and mucoadhesion properties of thiolated trimethyl chitosan nanoparticles in oral insulin delivery,” Biomaterials, vol. 30, no. 29, pp. 5691–5700, 2009. View at Publisher · View at Google Scholar · View at Scopus
  30. J. Hombach, H. Hoyer, and A. Bernkop-Schnürch, “Thiolated chitosans: development and in vitro evaluation of an oral tobramycin sulphate delivery system,” European Journal of Pharmaceutical Sciences, vol. 33, no. 1, pp. 1–8, 2008. View at Publisher · View at Google Scholar · View at Scopus
  31. L. M. Y. Yu, K. Kazazian, and M. S. Shoichet, “Peptide surface modification of methacrylamide chitosan for neural tissue engineering applications,” Journal of Biomedical Materials Research A, vol. 82, no. 1, pp. 243–255, 2007. View at Publisher · View at Google Scholar · View at Scopus
  32. M. Werle, B. Loretz, D. Entstrasser, and F. Föger, “Design and evaluation of a chitosan-aprotinin conjugate for the peroral delivery of therapeutic peptides and proteins susceptible to enzymatic degradation,” Journal of Drug Targeting, vol. 15, no. 5, pp. 327–333, 2007. View at Publisher · View at Google Scholar · View at Scopus
  33. D.-W. Lee, S. A. Shirley, R. F. Lockey, and S. S. Mohapatra, “Thiolated chitosan nanoparticles enhance anti-inflammatory effects of intranasally delivered theophylline,” Respiratory Research, vol. 7, article 112, 2006. View at Publisher · View at Google Scholar · View at Scopus
  34. I.-H. Bae, W. G. Jang, H.-P. Lim et al., “Morphological property and in vitro enzymatic degradation of modified chitosan as a scaffold,” Macromolecular Research, vol. 19, no. 12, pp. 1250–1256, 2011. View at Publisher · View at Google Scholar · View at Scopus
  35. A. F. S. A. Habeeb, “A sensitive method for localization of disulfide containing peptides in column effluents,” Analytical Biochemistry, vol. 56, no. 1, pp. 60–65, 1973. View at Google Scholar · View at Scopus
  36. R. B. Sashidhar, A. K. Capoor, and D. Ramana, “Quantitation of ε-amino group using amino acids as reference standards by trinitrobenzene sulfonic acid. A simple spectrophotometric method for the estimation of hapten to carrier protein ratio,” Journal of Immunological Methods, vol. 167, no. 1-2, pp. 121–127, 1994. View at Google Scholar · View at Scopus
  37. H. J. Schramm and T. Dülffer, “Synthesis and application of cleavable and hydrophilic crosslinking reagents,” Advances in Experimental Medicine and Biology, vol. 86, pp. 197–206, 1977. View at Google Scholar · View at Scopus
  38. H. Nazar, D. G. Fatouros, S. M. Van Der Merwe et al., “Thermosensitive hydrogels for nasal drug delivery: the formulation and characterisation of systems based on N-trimethyl chitosan chloride,” European Journal of Pharmaceutics and Biopharmaceutics, vol. 77, no. 2, pp. 225–232, 2011. View at Publisher · View at Google Scholar · View at Scopus
  39. K. Kataoka, Y. Suzuki, M. Kitada et al., “Alginate enhances elongation of early regenerating axons in spinal cord of young rats,” Tissue Engineering, vol. 10, no. 3-4, pp. 493–504, 2004. View at Publisher · View at Google Scholar · View at Scopus
  40. P. Prang, R. Müller, A. Eljaouhari et al., “The promotion of oriented axonal regrowth in the injured spinal cord by alginate-based anisotropic capillary hydrogels,” Biomaterials, vol. 27, no. 19, pp. 3560–3569, 2006. View at Publisher · View at Google Scholar · View at Scopus
  41. B. G. Keselowsky, L. Wang, Z. Schwartz, A. J. Garcia, and B. D. Boyan, “Integrin α5 controls osteoblastic proliferation and differentiation responses to titanium substrates presenting different roughness characteristics in a roughness independent manner,” Journal of Biomedical Materials Research A, vol. 80, no. 3, pp. 700–710, 2007. View at Publisher · View at Google Scholar · View at Scopus
  42. J. J. J. P. van den Beucken, X. F. Walboomers, O. C. Boerman et al., “Functionalization of multilayered DNA-coatings with bone morphogenetic protein 2,” Journal of Controlled Release, vol. 113, no. 1, pp. 63–72, 2006. View at Publisher · View at Google Scholar · View at Scopus