Figure 3: Identification of the response sequence to CREBH in the human APOA5 gene promoter. A series of deletion constructs of human APOA5 promoter luciferase reporters and a construct containing a mutation (cross) of the putative CREBH binding site (CREBHRE) were cotransfected with a plasmid expressing CREBH-N or the empty plasmid pcDNA3 as a control, into HepG2 cells. Luciferase activity was measured using the Dual-Glo Luciferase Assay System. Representative data from three independent experiments are shown. Statistical differences from controls are indicated by asterisks ( and ).