Molecular Prevalence of Babesia bigemina and Trypanosoma evansi in Dairy Animals from Punjab, India, by Duplex PCR: A Step Forward to the Detection and Management of Concurrent Latent Infections

Table 1

Oligonucleotide primers used for establishment of duplex PCR.

Haemoprotozoan	Primer	Region amplified	Product size
B. bigemina [10]	Bg3 5 ′ TAG TTG TAT TTC AGC CTC GCG 3 ′	Small subunit ribosomal RNA sequence of B. bigemina	689 bp
	Bg4 5 ′ AAC ATC CAA GCA GCT AHT TTA G 3 ′
T. evansi [11]	TR3 5 ′ GCG CGG ATT CTT TGC AGA CGA 3 ′	Repetitive nucleotide sequences of T. evansi	257 bp
	TR4 5 ′ TGC AGA CAC TGG AAT GTT ACT 3 ′