Molecular Prevalence of Babesia bigemina and Trypanosoma evansi in Dairy Animals from Punjab, India, by Duplex PCR: A Step Forward to the Detection and Management of Concurrent Latent Infections

Table 3

Correlation of infection of T. evansi, B. bigemina, and dual infection with sensitivity of PCR.

	T. evansi positive (150)			B. bigemina positive (10)			Mixed infection positive (14)
	+/CS	−/CS	−/NCS	+/CS	−/CS	−/NCS	+/CS	−/CS	−/NCS
PCR positive	3 (2%)	62 (41.33)	85 (56.6)	2 (20.0)	3 (30)	5 (50)	0	9 (64.85)	5 (35.71)

+/CS: sample positive by slide and animal showed clinical signs (clinical cases).
−/CS: sample negative by slide and animal showed clinical signs (subclinical cases).
−/NCS: sample negative by slide and animal showed no clinical signs (latent carriers).