Rapid Purification and Procoagulant and Platelet Aggregating Activities of Rhombeobin: A Thrombin-Like/Gyroxin-Like Enzyme from Lachesis muta rhombeata Snake Venom

Figure 5

“In vitro” (a) activated partial thromboplastin time (APPT) and (b) prothrombin time (PT) blood tests of PBS (■) or different quantities of Rhombeobin (●) on mice citrated platelet poor plasma. In vivo time course effect on (c) APPT and (d) PT blood tests of male adult mice after i.v. injection of Rhombeobin (●) control mice received i.v injection of PBS (■) only. Results are expressed in seconds. Error bars indicate mean plus or minus standard deviation: “in vitro” (𝑛=3 for each group) and “ex vivo” (𝑛=5 for each group). Error bars indicate mean plus or minus SME (𝑛=5); * means a significant difference (𝑃<0.05) compared with the control.

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