Table 1: Cause for sequence-dependent off-target effects.

#Causes for off-target effectsReferences

1Nearly exact complementarity[8, 10]
215 nt in total or even 11 continuous nt match[8]
3Seed region complementarity[9, 12, 15]
4miRNA function (seed region-3UTR conserved region complementarity)[13]
5Multiple occurrences of the seed region in an mRNA sequence[13, 15]
6Complementary region at the cleavage site, center of the siRNA[11, 16]
7Tolerance of G:U wobble[16]
8Seed complementation frequency[12]
9High G/C content in the seed region[15]