Responses of Growth Performance and Proinflammatory Cytokines Expression to Fish Oil Supplementation in Lactation Sows’ and/or Weaned Piglets’ Diets

Figure 1

Relative longissimus dorsi muscle and spleen cytokines expression of weaned piglets born to fish oil-treated or control sows, and piglets fed diets with and without fish oil supplementation during the postweaning period. (a), (b), and (c) were longissimus dorsi muscle cytokines expression; (d), (e), and (f) were spleen cytokines expression. (a) and (d): IL-1β expression; (b) and (e): IL-6 expression; (c) and (f): TNF-α expression. Gene signals were normalized to the expression of the housekeeping gene β-actin optical density signal. CC: all period fed lard diet; CT: postweaning period fed fish oil diet; TC: lactation period fed fish oil diet; TT: all period fed fish oil diet. Data shown are mean values ±SEM, 𝑛=4. ^*,**: means lactation decrease effect with 𝑃<0.05, 𝑃<0.01; ^†,‡: means postweaning increase effect with 𝑃<0.05, 𝑃<0.01; a,b: means with different letters are significantly different (𝑃<0.05) among groups; nt: means have no significant difference.

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