Inflammatory Bowel Disease Therapies and Gut Function in a Colitis Mouse Model
Figure 3
TEER of intestinal mucosa of male and female C57BL/6 mice. Mice from each group were euthanized either 2 weeks (a) or 4 weeks (3 weeks in HC group) (b) following treatment, and colon tissue was collected. Tissue segments were opened along the mesenteric border and then cut into flat sheet and placed into the Ussing chamber with TEER measured. H. trogontum significantly decreased TEER in the infected controls (weeks 2 and 4: versus noninfected controls for both comparisons). In contrast, the EEN, MNZ, and EEN + MNZ treatments remarkably ameliorated the increased TEER observed in infected controls (weeks 2 and 4: versus noninfected controls for all comparisons). The group of mice treated with HC had TEER levels below the noninfected controls (weeks 2 and 3: for both comparisons) but elevated slightly but not significantly compared with the infected controls (week 2 and 3: for both comparisons). Bars indicate mean. NS: not significant; . TEER: transepithelial electrical resistance; HC: hydrocortisone; EEN: exclusive enteral nutrition; MNZ: metronidazole.
