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BioMed Research International
Volume 2013 (2013), Article ID 910459, 6 pages
Research Article

Cytotoxicity Testing of Temporary Luting Cements with Two- and Three-Dimensional Cultures of Bovine Dental Pulp-Derived Cells

1Department of Restorative Dentistry, Faculty of Dentistry, Selcuk University, 42075 Konya, Turkey
2Department of Prosthodontics, Faculty of Dentistry, Erciyes University, 38039 Kayseri, Turkey
3Department of Restorative Dentistry, Faculty of Dentistry, Inonu University, 44280 Malatya, Turkey
4Department of Restorative Dentistry, Faculty of Dentistry, Kırıkkale University, 71100 Kırıkkale, Turkey

Received 19 April 2013; Accepted 8 July 2013

Academic Editor: Andre Van Wijnen

Copyright © 2013 Hayriye Esra Ülker et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study evaluated the cytotoxicity of eugenol-containing and eugenol-free temporary luting cements. For cytotoxicity testing, bovine pulp-derived cells transfected with Simian virus 40 Large T antigen were exposed to extracts of eugenol-containing (Rely X Temp E) and eugenol-free (Provicol, PreVISION CEM, and Rely X Temp NE) temporary luting cements for 24 h. The cytotoxicity of the same materials was also evaluated in a dentin barrier test device using three-dimensional cell cultures of bovine pulp-derived cells. The results of the cytotoxicity studies with two-dimensional cultures of bovine dental pulp-derived cells revealed that cell survival with the extracts of Rely X Temp E, Provicol, PreVISION CEM, and Rely X Temp NE was 89.1%, 84.9%, 92.3%, and 66.8%, respectively. Rely X Temp NE and Provicol showed cytotoxic effects on bovine dental pulp-derived cells ( ). The results of the dentin barrier test revealed that cell survival with the above-mentioned temporary cement was 101.5%, 91.9%, 93.5%, and 90.6%, respectively. None of the temporary luting cements significantly reduced cell survival compared with the negative control in the dentin barrier test ( ). Biologically active materials released from temporary luting cements may not influence the dentine-pulp complex if the residual dentine layer is at least 0.5 mm thick.