Mouse Prostate Epithelial Luminal Cells Lineage Originate in the Basal Layer Where the Primitive Stem/Early Progenitor Cells Reside: Implications for Identifying Prostate Cancer Stem Cells
Figure 5
In the basal layer of transgenic prostates, H2BGFP-labeled, slowly cycling cells are keratin 5-specific, not keratin 18-specific. (a) Fluorescent microscopy confirmation of H2BGFP tet-off labeling and chase in the prostate of the double transgenic mice. Mouse tissues containing H2BGFP-labeled, slowly cycling cells, costained with keratin 5 antibody, were specific to the basal layer. (b) The H2BGFP label in mouse tissue was confirmed by a GFP-specific antibody. (c) In the transgenic prostate, H2BGFP-labeled, slowly cycling cells were not keratin 18-specific, as determined by costaining with keratin 18 antibody.
