Figure 4: Cytotoxic effect of B. variegata leaf extracts against cancer cell lines using SRB assay. Percentage growth inhibition of DU-145 (prostate), HOP-62 (lung), IGR-OV-1 (ovary), MCF-7 (breast), and THP-1 (leukemia) cancer cell lines was assayed at 100 μg/mL concentration of extracts as described in Section 2. Abbreviations: PE—petroleum ether, BZ—benzene, CH—chloroform, EA—ethyl acetate, AC—acetone, ET—ethanol, and AQ—water. ACD—Anticancer drugs (mitomycin-C (10 μM) against prostate, Paclitaxel (10 μM) against lung and breast, Adriamycin (1 μM) against ovary and 5-Flurouracil (20 μM) against leukemia human cancer cell lines). Data represent mean ± SD of three replicates ( ).