Transcriptional Profiling of Canker-Resistant Transgenic Sweet Orange (<i>Citrus sinensis</i> Osbeck) Constitutively Overexpressing a Spermidine Synthase Gene

<table>Validation of microarray results by semiquantitative RT-PCR. Ten probes putatively encoding lipid transfer protein (Cit.60.1.S1_at), glutathione s-transferase (Cit.6308.1.S1_at, Cit.9510.1.S1_s_at), ABC transporter ATPase (Cit.3246.1.S1_at), phospholipase d (Cit.6097.1.S1_s_at), cell wall invertase (Cit.5734.1.S1_at), miraculin-like protein 2 (Cit.57.1.S1_at) and unknown protein (Cit.19313.1.S1_at, Cit.5367.1.S1_at, Cit.1406.1.S1_s_at) were amplified with specific primers in WT and TG9 leaves, using <i>Actin</i> gene as an internal control for examining equal cDNA loading. The expression ratios between TG9 and WT were calculated by quantifying the band density using the Quantity One software.</table>

BioMed Research International

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Figure 1

Figure 1: Transcriptional Profiling of Canker-Resistant Transgenic Sweet Orange (<i>Citrus sinensis</i> Osbeck) Constitutively Overexpressing a Spermidine Synthase Gene 

Figure 1 | Transcriptional Profiling of Canker-Resistant Transgenic Sweet Orange (Citrus sinensis Osbeck) Constitutively Overexpressing a Spermidine Synthase Gene 