Transcriptional Profiling of Canker-Resistant Transgenic Sweet Orange (Citrus sinensis Osbeck) Constitutively Overexpressing a Spermidine Synthase Gene

Figure 3

Expression of candidate genes in leaves of WT and TG9 infected or not with Xanthomonas citri subsp. Citri (Xcc). Eleven candidate genes putatively encoding cysteine proteinase inhibitor (Cit.8163.1.S1_x_at), thaumatin-like protein (Cit.11548.1.S1_at), cytochrome p450 (Cit.4425.1.S1_at), aspartyl protease family protein (Cit.28117.1.S1_s_at), pyruvate kinase (Cit.13055.1.S1_at), pathogenesis-related protein (Cit.20495.1.S1_at), thioredoxin-like 5 (Cit.5856.1.S1_at), AP2/ERF transcription factor (Cit.17124.1.S1_at), NADPH oxidase (Cit.2333.1.S1_at), TIS-NBS-LRR resistance protein (Cit.6121.1.S1_at), and Rubisco subunit binding protein (Cit.31932.1.S1_at) were assessed at 0 and 24 h post inoculation (hpi) in WT and TG9 leaves via semiquantitative RT-PCR. Column diagram was the quantification data of corresponding bands using Quantity One software.