Actin, RhoA, and Rab11 Participation during Encystment in Entamoeba invadens
Figure 3
Polymerized actin levels vary along the encystation process. (a) Micrographs showing total (anti-actin antibody 1 : 10,000 followed by a secondary FITC-conjugated goat anti-mouse IgG antibody, 1 : 1,000), and filamentous actin (Rhodamine phalloidin, 1 : 50) in the three developmental stages during encystment. (b) Graph representing the intensity of T-actin and F-actin. T-actin decreases in precyst and cysts, but F-actin increased in precysts. Results are the average of three independent experiments done in duplicate (, ).
