Figure 3: Uterus transverse section (a), wet weight of uterine tissue (b), and serum 17β-estradiol level (c) of ovariectomized (OVX) mice treated with milk thistle extract (MTE) and silibinin. OVX mice were orally treated with 10 mg/kg/day MTE or 10 mg/kg/day silibinin daily for 8 weeks. After being washed with saline, uterine tissues were fixed in 10% neutral buffered formalin for 24 h. Cross-sectional images of uterine horn were obtained by staining with hematoxylin and eosin and visualized under light microscopy (3 separate experiments). Magnification: 40-fold. Serum 17β-estradiol level was determined by using assay kits. Values in bar graphs (mean ± SEM, ) not sharing a letter indicate significant difference at .