Green Tea Polyphenol Epigallocatechin-3-Gallate Enhance Glycogen Synthesis and Inhibit Lipogenesis in Hepatocytes

Figure 2

Glycogen synthesis in response to GTP-EGCG treatments in HepG2 cells. The cells were pretreated with high glucose (30 mM) for 24 h then incubated with various concentrations of EGCG (0.1–10 μM) with 100 nM insulin for 24 h. HepG2 cultured with high glucose without insulin was used to confirm that the HepG2 cells became insulin resistant. 1 μCi ¹⁴C-glucose solution was added to HepG2 cells for 30 min at 37°C. The results were attained by measuring the rate of incorporation of D-[U-¹⁴C]glucose into glycogen. Data are means ± S.E. The data are from five separate experiments. *𝑃<0.05 and **𝑃<0.01 compared with insulin stimulation alone.