Green Tea Polyphenol Epigallocatechin-3-Gallate Enhance Glycogen Synthesis and Inhibit Lipogenesis in Hepatocytes
Figure 3
Effects of dose response to GTP-EGCG on lipogenesis in HepG2 cells. HepG2 cells were stained with oil Red O solution, and the dye was extracted from lipid droplets from cells. Images of cells were captured by microscope at 20x original magnification showing lipid accumulation in cells stained by Oil Red O. (A) HepG2 cells cultured in 30 mM glucose, (B) HepG2 cells cultured in 30 mM glucose with 100 nM insulin stimulation, and (C) HepG2 cells cultured in 30 mM glucose with 100 nM insulin stimulation and 10 μM of GTP/EGCG. The degree of lipogenesis was quantified from lipid droplets in cells by measuring the absorbance at 490 nm. The changes of lipogenesis by EGCG treatments were calculated as the percentage of insulin stimulation alone. Data are means ± S.E from five independent experiments. * and * compared with insulin treatment alone.
