CpG and Interleukin-15 Synergize to Enhance IFN-γ Production by Activated CD8+ T Cells
Figure 3
IL-12 is required for CpG+IL-15-mediated enhancement of IFN- production. (a) Splenocytes were harvested from the spleens of uninfected (filled bars) or T. cruzi-infected wild-type (open bars) mice on day 9 p.i. Thy1.2-depleted splenocytes were then cultured in the presence of IL-15, CpG, or both IL-15 and CpG. After 72 hours, culture supernatants were harvested and tested for IL-12p40 by ELISA. (b) CD8+ T cells and live Thy1.2-depleted APC were cocultured with anti-CD3 for 72 hours in the presence of IL-15, CpG, IL-15+CpG, IL-15+CpG+isotype antibody, or IL-15+CpG+anti-IL-12p40 antibody. Cell culture supernatants were then harvested and IFN- levels were determined by ELISA. (c) Splenocytes from wild-type (filled bars) or IL-12p35−/− (open bars) mice infected with T. cruzi were cultured for 72 hours with Tskb20 peptide. **; *; NS: not significant. Results are representative of three to four independent experiments with at least two mice per group and expressed as .
