Research Article
Structural Adaptation of Cold-Active RTX Lipase from Pseudomonas sp. Strain AMS8 Revealed via Homology and Molecular Dynamics Simulation Approaches
Table 1
Ramachandran plot scores for LipAMS8 after simulation for 12 ns at different temperatures.
| Temperature | 0°C | 5°C | 25°C | 37°C | 50°C | 100°C |
| Most favored regions (%) | 85.1 | 83.1 | 87.2 | 81.3 | 84.4 | 82.1 | Additional allowed regions (%) | 13.1 | 15.9 | 12.3 | 17.7 | 14.9 | 16.2 | Generously allowed regions (%) | 1.5 | 0.8 | 0.3 | 0.3 | 0.0 | 1.3 | Disallowed regions (%) | 0.3 | 0.3 | 0.3 | 0.8 | 0.8 | 0.5 | Nonglycine and nonproline residues (%) | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 | Endresidues (excl. Gly and Pro) | 2 | 2 | 2 | 2 | 2 | 2 | Glycine residues | 69 | 69 | 69 | 69 | 69 | 69 | Proline residues | 15 | 15 | 15 | 15 | 15 | 15 | Total number of residues | 476 | 476 | 476 | 476 | 476 | 476 |
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