927643.fig.003a
(a)
927643.fig.003b
(b)
927643.fig.003c
(c)
927643.fig.003d
(d)
Figure 3: Effects of sodium octanoate on the expression of antimicrobial peptide mRNA. qPCR analysis that shows the effect on TAP (a), BNBD5 (b), DEFB1 (c), and S100A7 (d) mRNA expression. bMECs were treated with the NaO concentrations (mM) indicated (24 h) and then were challenged with S. aureus during 2 h. Each bar shows the mean of triplicates ± SE of three independent experiments. GAPDH was used as endogenous gene in all conditions. The symbol “*” indicates significant changes ( ) in relation to unchallenged bMEC within each treatment. The letter “a” indicates significant changes ( ) compared to control cells without treatment. The letter “b” indicates significant changes ( ) compared to challenged bMEC.