930686.fig.002
Figure 2: Effects of reaction time and temperature on biodiesel yield by in situ transesterification of wet Chlorella pyrenoidosa biomass. Reaction conditions: 100 mg dry weight equivalent wet biomass (oil content 47%, water content 90%) with 4 mL methanol, 6 mL n-hexane, and 0.5 M sulfuric acid. Reaction time: 90, 120, 180, 240 and 300 min. Temperature: 90, 120, and 150°C.