Direct Biodiesel Production from Wet Microalgae Biomass of Chlorella pyrenoidosa through In Situ Transesterification

Table 2

FAME contents of the biodiesels from wet Chlorella pyrenoidosa biomass under different temperature and different reaction time by in situ transesterification. Reaction conditions: 100 mg dry weight equivalent wet biomass (oil content 47%, water content 90%) with 4 mL methanol, 6 mL n-hexane, and 0.5 M sulfuric acid.

Reaction time (min)	FAME content (%)
	90°C	120°C	150°C
90	90.76	92.89	89.75
120	87.63	91.78	90.56
180	90.14	92.14	90.17
240	89.39	90.27	88.26
300	90.57	89.85	87.92