Figure 1: Fluorescence microscopy of NF-200 and ChAT stainings in different nerves. Costaining for neurofilament (NF-200, green) and for Choline-Acetyl-Transferase (ChAT, red). The saphenous nerve (g–i) as well as the lateral femoral cutaneous nerve as negative control for ChAT (a–c) contains no motor neurons and was therefore clearly negative for ChAT. In contrast to the saphenous nerve, the obturator nerve (k–m) as well as the sciatic nerve as positive control (d–f) contains ChAT-positive motor neurons. Scale bar: 50 μm.