Figure 5: Expression of neurotensin and neurotensin receptors, NTR1, NTR2, and NTR3 in macrophages at 10 and 30 mM glucose, by real-time PCR. Cells were plated at 2 × 106/well and maintained at the indicated conditions. Total RNA was isolated as described in “Materials and Methods.” The relative gene expression is indicated as arbitrary units and was obtained after normalization with the HPRT gene. Results are presented as mean ± SEM of five to eight independent experiments. .