Figure 6: Expression of neurotensin and neurotensin receptors, NTR1, NTR2, and NTR3 in macrophages at 10 and 30 mM glucose, by real-time PCR. Cells were plated at 2 × 106/well and treated with 10 nM NT or 1 μM/mL LPS or both, during 6 h. Total RNA was isolated as described in “Materials and Methods.” The relative gene expression is indicated as arbitrary units and was obtained after normalization with the HPRT gene. Results are presented as mean ± SEM of six to nine independent experiments. ; .