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BioMed Research International
Volume 2013, Article ID 946268, 5 pages
http://dx.doi.org/10.1155/2013/946268
Research Article

Hypomethylation of ETS Transcription Factor Binding Sites and Upregulation of PARP1 Expression in Endometrial Cancer

1Department of Obstetrics and Gynecology, Shengjing Hospital, China Medical University, Shenyang 110004, China
2Department of Physiology, Institute of Basic Medical Sciences, China Medical University, Shenyang 110001, China

Received 4 February 2013; Accepted 22 April 2013

Academic Editor: Andre Van Wijnen

Copyright © 2013 Fang-Fang Bi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Although PARP1 promoter methylation is involved in the regulation of PARP1 expression in human keratinocyte lines and lymphoblastoid cell lines, its roles in human endometrial cancer are unknown. DNA from forty normal endometrium (NE) and fifty endometrial adenocarcinoma (EAC) tissues were analyzed by bisulfite sequencing using primers focusing on the core promoter region of PARP1. Expression levels of PARP1 were assessed by immunohistochemistry and real-time PCR. Associations between patient clinicopathological characteristics and PARP1 protein levels were assessed by Fisher’s exact test. Here, PARP1 mRNA and protein were overexpressed in EAC tissues . CpG sites within the ETS motif in the PARP1 promoter exhibited significant hypomethylation in EAC tissues, and there was a significant negative correlation between PARP1 mRNA levels and the number of methylated sites in both NE and EAC tissues ( , ). Notably, PARP1 protein expression was associated with FIGO stage , histological grade , and body mass index . Our findings imply that PARP1 overexpression may participate in endometrial cancer progression, and abnormal hypomethylation of CpG sites within the ETS motif in the core promoter region may be responsible for PARP1 overexpression in EAC tissues.