Figure 3: In vitro monolayer scratch cell migration assay. Confluent cells were treated with different concentration of HE (0, 0.1, 0.25, 0.5, 0.75, and 1.0 mg/mL) and also beraprost (50 μg/mL) for 16 h time duration. Distance travelled from wound edge to the highest cell point was calculated in mm. Black line indicates the wound edge.