Figure 5: RT-PCR and WB analysis. (a) Western blot analysis and semiquantitative reverse transcription analysis of Rac-1, Rho-A, Cdc-42, Cdk-1, Cdk-2, and β-actin. Cells were treated with different concentration of HE (0, 0.1, 0.25, 0.5, 0.75, and 1.0 mg/mL) and processed for RT-PCR and W.B analysis for genes (right panel) and proteins (left panel), respectively. Data here represents the picture from one of three independent experiments. (b) Relative values of mRNA and protein expression which are normalised against β-actin.