Rotational Transport of Islets: The Best Way for Islets to Get around?
Islet cell viability and cell stress assessed by live confocal microscopy. (a) Cells imaged immediately after isolation and stained with WGA to assess cell morphology and with TMRM to detect cell viability. (b) Cells imaged after 15 h simulated transport under perfused rotary conditions; WGA and TMRM were used as dyes. (c) Cell morphology (WGA) and viability of islet cells after 15 h simulated transport under static conditions. (d) Islets analyzed immediately after isolation and stained with WGA for cell morphology and with Rhod-2 for the assessment of cell stress. (e), (f) Cell morphology and islet stress assessed after 15 h simulated transport under rotary versus static conditions.