Single Nucleotide Polymorphism in the Promoter of the Human Interleukin-13 Gene Is Associated with Asthma in Malaysian Adults

Table 1

Technical data for the analysis of single nucleotide polymorphisms (SNPs) in the human IL-13 gene.

Region	Primers		PCR product size (bp)	Restriction enzyme*	Annealing temperature	Reference
Promoter: (−1111C>T)	Forward: Reverse:	5′-ACT TCT GGG AGT CAG AGC CA-3′; 5′-TAC AGC CAT GTC GCC TTT TCC TGC TCT TCC GTC-3′	372	Hpy99I	60°C	[22]
Coding: (4257G>A)	Forward: Reverse:	5′-CTT CCG TGA GGA CTG AAT GAG ACG GTC-3′; 5′-GCA AAT AAT GAT GCT TTC GAA GTT TCA GTG GA-3′	236	NlaIV	60°C	[23]

GenBank accession number U31120 was used as the reference sequence.
*New England Biolabs, Beverly, MA, USA.