Antibacterial Activity of Defensin PaDef from Avocado Fruit (<i>Persea americana</i> var. <i>drymifolia</i>) Expressed in Endothelial Cells against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>

<table>Transfection of BVE-E6E7 endothelial cells with pBME3 construction. (a) The pBME3 construction contains the EF1-<svg height="7.9499998" id="M11" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 9.7749996 7.9499998" width="9.7749996" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,7.675)"><path d="M545 106q-67 -118 -134 -118q-24 0 -40 37.5t-30 129.5h-2q-47 -72 -103 -119.5t-108 -47.5q-47 0 -76 45.5t-29 119.5q0 113 85 204t174 91q47 0 70 -33.5t43 -119.5h3q32 47 80 140l55 13l10 -9q-47 -80 -138 -201q17 -99 27.5 -136t22.5 -37q23 0 69 61zM333 204
q-14 98 -31 149.5t-50 51.5q-49 0 -94 -70t-45 -164q0 -55 15.5 -86t40.5 -31q70 0 164 150z" id="x1D6FC"></path></g>
</svg> promoter, defensin <i>PaDef </i>cDNA, CMV promoter, EM7 promoter, GFP-zeocin resistance gene, and SV40 polyadenylation sequence. (b) Polyclonal, population (BVE3-PC) and representative cloned population (BVE3-C1) of BVE-E6E7 cells transfected with pBME3 visualized under light microscopy (upper) and fluorescence (below). Also, the control cells are showed (BVpT). Scale bar: 100 <i>μ</i>m.</table>

BioMed Research International

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Figure 2

Figure 2: Antibacterial Activity of Defensin PaDef from Avocado Fruit (<i>Persea americana</i> var. <i>drymifolia</i>) Expressed in Endothelial Cells against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> 

Figure 2 | Antibacterial Activity of Defensin PaDef from Avocado Fruit (Persea americana var. drymifolia) Expressed in Endothelial Cells against Escherichia coli and Staphylococcus aureus 