Antibacterial Activity of Defensin PaDef from Avocado Fruit (Persea americana var. drymifolia) Expressed in Endothelial Cells against Escherichia coli and Staphylococcus aureus
Figure 4
Defensin PaDef expression in BVE-E6E7 endothelial cells analyzed by flow cytometry. (a) Left: scatter plot of clonal population of BVE-E6E7 cells transfected with BVpT (control cells). Right: scatter plot of clonal population of BVE-E6E7 cells that express the defensin PaDef (BVE3-C1). The values inside rectangle indicate the percentage of cells that displayed fluorescence intensity higher than 2000 relative units. (b) Relative fluorescence intensity of BVE-E6E7 control cells (BVpT) and BVE-E6E7 cells that express the defensin PaDef (BVE3-C1). Fluorescence intensity was estimated from 5500 events. A control of BVE-E6E7 cells that express the defensin PaDef (BVE3-C1) only treated with the secondary antibody was included (BVE3-C1 + secondary Ab). Values of relative fluorescence intensity were obtained subtracting the fluorescence intensity corresponding to GFP expression. *Significant changes () compared to BVE-E6E7 control cells.
