Protective Effects of Testosterone on Presynaptic Terminals against Oligomeric -Amyloid Peptide in Primary Culture of Hippocampal Neurons

Figure 3

Testosterone attenuated oligomeric Aβ-induced reduction of synaptotagmin in primary hippocampal neurons. Primary hippocampal neurons were treated with 10 nM testosterone for 1 h, followed by exposure to 5 μM oligomeric Aβ for 24 h. Neurons were stained with synaptotagmin antibody. (a) Control, (b) 10 nM testosterone for 25 h, (c) 5 μM Aβ for 24 h, and (d) 10 nM testosterone for 1 h, followed by exposure to 5 μM Aβ for 24 h. (e) The number of puncta and (f) the fluorescent intensity were analyzed by Image J software as described in the above section. ^∗𝑃<0.05 versus control group, ^∗∗𝑃<0.005 versus control group, and ^##𝑃<0.005 versus Aβ group.

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