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BioMed Research International
Volume 2014, Article ID 108913, 11 pages
http://dx.doi.org/10.1155/2014/108913
Research Article

Lipolytic Potential of Aspergillus japonicus LAB01: Production, Partial Purification, and Characterisation of an Extracellular Lipase

1Cell Signaling, Nanobiotechnology and Enzymology Laboratory, Federal University of Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil
2Laboratory of Applied Microbiology, Federal University of Minas Gerais 31270-901, Belo Horizonte, MG, Brazil

Received 16 May 2014; Revised 31 August 2014; Accepted 2 September 2014; Published 29 October 2014

Academic Editor: Yunjun Yan

Copyright © 2014 Lívia Tereza Andrade Souza et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. A. Maller, A. R. L. Damasio, T. M. Silva, J. A. Jorge, H. F. Terenzi, and M. L. T. M. Polizeli, “Potential application in animal feed of phytase produced from agro-industrial residues by Aspergillus japonicus,” Journal of Biotechnology, vol. 150, p. 514, 2010. View at Google Scholar
  2. F. D. A. Facchini, A. C. Vici, V. R. A. Reis et al., “Production of fibrolytic enzymes by Aspergillus japonicus CO3 using agro-industrial residues with potential application as additives in animal feed,” Bioprocess and Biosystems Engineering, vol. 34, no. 3, pp. 347–355, 2011. View at Publisher · View at Google Scholar · View at Scopus
  3. M. V. Semenova, S. G. Grishutin, A. V. Gusakov, O. N. Okunev, and A. P. Sinitsyn, “Isolation and properties of pectinases from the fungus Aspergillus japonicus,” Biochemistry, vol. 68, no. 5, pp. 559–569, 2003. View at Publisher · View at Google Scholar · View at Scopus
  4. F. D. A. Facchini, A. C. Vici, V. M. Benassi et al., “Optimization of fibrolytic enzyme production by Aspergillus japonicus C03 with potential application in ruminant feed and their effects on tropical forages hydrolysis,” Bioprocess and Biosystems Engineering, vol. 34, no. 8, pp. 1027–1038, 2011. View at Publisher · View at Google Scholar · View at Scopus
  5. M. Wakiyama, K. Yoshihara, S. Hayashi, and K. Ohta, “An extracellular endo-1,4-β-xylanase from Aspergillus japonicus: purification, properties, and characterization of the encoding gene,” Journal of Bioscience and Bioengineering, vol. 109, no. 3, pp. 227–229, 2010. View at Publisher · View at Google Scholar · View at Scopus
  6. S. I. Mussatto, C. N. Aguilar, L. R. Rodrigues, and J. A. Teixeira, “Colonization of Aspergillus japonicus on synthetic materials and application to the production of fructooligosaccharides,” Carbohydrate Research, vol. 344, no. 6, pp. 795–800, 2009. View at Publisher · View at Google Scholar · View at Scopus
  7. R. Sharma, Y. Chisti, and U. C. Banerjee, “Production, purification, characterization, and applications of lipases,” Biotechnology Advances, vol. 19, no. 8, pp. 627–662, 2001. View at Publisher · View at Google Scholar · View at Scopus
  8. K. Tongboriboon, B. Cheirsilp, and A. H-Kittikun, “Mixed lipases for efficient enzymatic synthesis of biodiesel from used palm oil and ethanol in a solvent-free system,” Journal of Molecular Catalysis B: Enzymatic, vol. 67, no. 1-2, pp. 52–59, 2010. View at Publisher · View at Google Scholar · View at Scopus
  9. F. Hasan, A. A. Shah, and A. Hameed, “Industrial applications of microbial lipases,” Enzyme and Microbial Technology, vol. 39, no. 2, pp. 235–251, 2006. View at Publisher · View at Google Scholar · View at Scopus
  10. F. J. Contesini, D. B. Lopes, G. A. MacEdo, M. D. G. Nascimento, and P. D. O. Carvalho, “Aspergillus sp. lipase: potential biocatalyst for industrial use,” Journal of Molecular Catalysis B: Enzymatic, vol. 67, no. 3-4, pp. 163–171, 2010. View at Publisher · View at Google Scholar · View at Scopus
  11. M. M. R. Talukder, H. Z. S. Lee, R. F. Low, L. C. Pei-Lyn, D. Warzecha, and J. Wu, “Potential use of whole cell lipase from a newly isolated Aspergillus nomius for methanolysis of palm oil to biodiesel,” Journal of Molecular Catalysis B: Enzymatic, vol. 89, pp. 108–113, 2013. View at Publisher · View at Google Scholar · View at Scopus
  12. N. Z. Adham and E. M. Ahmed, “Extracellular lipase of Aspergillus niger NRRL3; production, partial purification and properties,” Indian Journal of Microbiology, vol. 49, no. 1, pp. 77–83, 2009. View at Publisher · View at Google Scholar · View at Scopus
  13. R. K. Saxena, W. S. Davidson, A. Sheoran, and B. Giri, “Purification and characterization of an alkaline thermostable lipase from Aspergillus carneus,” Process Biochemistry, vol. 39, no. 2, pp. 239–247, 2003. View at Publisher · View at Google Scholar · View at Scopus
  14. Y. Kaminishi, H. Tanie, and M. Kunimoto, “Purification and characterization of lipases from Aspergillus repens and Eurotium herbariorum NU-2 used in “Katsuobushi” molding,” Fisheries Science, vol. 65, no. 2, pp. 274–278, 1999. View at Google Scholar · View at Scopus
  15. R. P. Yadav, R. K. Saxena, R. Gupta, and W. S. Davidson, “Purification and characterization of a regiospecific lipase from Aspergillus terreus,” Biotechnology and Applied Biochemistry, vol. 28, no. 3, pp. 243–249, 1998. View at Google Scholar · View at Scopus
  16. R. Gulati, R. K. Saxena, and R. Gupta, “Fermentation waste of Aspergillus terreus: a promising copper bio-indicator,” Current Science, vol. 77, no. 10, pp. 1359–1360, 1999. View at Google Scholar
  17. K. Ohnishi, Y. Yoshida, J. Toita, and J. Sekiguchi, “Purification and characterization of a novel lipolytic enzyme from Aspergillus oryzae,” Journal of Fermentation and Bioengineering, vol. 78, no. 6, pp. 413–419, 1994. View at Publisher · View at Google Scholar · View at Scopus
  18. H. Chander, V. K. Batish, S. S. Sannabhadti, and R. A. Srinivasan, “Factors affecting lipase production in Aspergillus wentii,” Journal of Food Science, vol. 45, no. 3, pp. 598–600, 1980. View at Google Scholar
  19. Y. Teng and Y. Xu, “A modified para-nitrophenyl palmitate assay for lipase synthetic activity determination in organic solvent,” Analytical Biochemistry, vol. 363, no. 2, pp. 297–299, 2007. View at Publisher · View at Google Scholar · View at Scopus
  20. E. Lorençon, A. S. Ferlauto, S. De Oliveira et al., “Direct production of carbon nanotubes/metal nanoparticles hybrids from a redox reaction between metal ions and reduced carbon nanotubes,” ACS Applied Materials and Interfaces, vol. 1, no. 10, pp. 2104–2106, 2009. View at Publisher · View at Google Scholar · View at Scopus
  21. S. L. Da Silva, J. R. Almeida, L. M. Resende et al., “Isolation and characterization of a natriuretic peptide from crotalus oreganus abyssus (grand canyon rattlesnake) and its effects on systemic blood pressure and nitrite levels,” International Journal of Peptide Research and Therapeutics, vol. 17, no. 3, pp. 165–173, 2011. View at Publisher · View at Google Scholar · View at Scopus
  22. U. K. Laemmli, “Cleavage of structural proteins during the assembly of the head of bacteriophage T4,” Nature, vol. 227, no. 5259, pp. 680–685, 1970. View at Publisher · View at Google Scholar · View at Scopus
  23. V. Paschon, G. S. V. Higa, R. R. Resende, L. R. G. Britto, and A. H. Kihara, “Blocking of connexin-mediated communication promotes neuroprotection during acute degeneration induced by mechanical trauma,” PLoS ONE, vol. 7, no. 9, Article ID e45449, 2012. View at Publisher · View at Google Scholar · View at Scopus
  24. E. S. de Alvarenga, S. A. Silva, L. C. A. Barosa et al., “Synthesis and evaluation of sesquiterpene lactone inhibitors of phospholipase A2 from Bothrops jararacussu,” Toxicon, vol. 57, no. 1, pp. 100–108, 2011. View at Publisher · View at Google Scholar · View at Scopus
  25. R. Singh, N. Gupta, V. K. Goswami, and R. Gupta, “A simple activity staining protocol for lipases and esterases,” Applied Microbiology and Biotechnology, vol. 70, no. 6, pp. 679–682, 2006. View at Publisher · View at Google Scholar · View at Scopus
  26. M.-A. Kwon, H. S. Kim, D.-H. Hahm, and J. K. Song, “Synthesis activity-based zymography for detection of lipases and esterases,” Biotechnology Letters, vol. 33, no. 4, pp. 741–746, 2011. View at Publisher · View at Google Scholar · View at Scopus
  27. C. M. Romero, L. M. Pera, C. Olivaro, A. Vazquez, and M. D. Baigori, “Tailoring chain length selectivity of a solvent-tolerant lipase activity from Aspergillus niger MYA 135 by submerged fermentation,” Fuel Processing Technology, vol. 98, pp. 23–29, 2012. View at Publisher · View at Google Scholar · View at Scopus
  28. M. S. M. Ali, C. C. Yun, A. L. T. Chor, R. N. Z. R. A. Rahman, M. Basri, and A. B. Salleh, “Purification and characterisation of an F16L mutant of a thermostable lipase,” Protein Journal, vol. 31, no. 3, pp. 229–237, 2012. View at Publisher · View at Google Scholar · View at Scopus
  29. J.-J. Shangguan, Y.-Q. Liu, F.-J. Wang et al., “Expression and characterization of a novel lipase from aspergillus fumigatus with high specific activity,” Applied Biochemistry and Biotechnology, vol. 165, no. 3-4, pp. 949–962, 2011. View at Publisher · View at Google Scholar · View at Scopus
  30. V. M. G. Lima, N. Krieger, D. A. Mitchell, and J. D. Fontana, “Activity and stability of a crude lipase from Penicillium aurantiogriseum in aqueous media and organic solvents,” Biochemical Engineering Journal, vol. 18, no. 1, pp. 65–71, 2004. View at Publisher · View at Google Scholar · View at Scopus
  31. A. Hiol, M. D. Jonzo, N. Rugani, D. Druet, L. Sarda, and L. C. Comeau, “Purification and characterization of an extracellular lipase from a thermophilic Rhizopus oryzae strain isolated from palm fruit,” Enzyme and Microbial Technology, vol. 26, no. 5-6, pp. 421–430, 2000. View at Publisher · View at Google Scholar · View at Scopus
  32. C. M. F. Soares, H. F. de Castro, F. F. de Moraes, and G. M. Zanin, “Characterization and utilization of Candida rugosa lipase immobilized on controlled pore silica,” Applied Biochemistry and Biotechnology, vol. 77–79, pp. 745–757, 1999. View at Google Scholar · View at Scopus
  33. S. Hama, S. Tamalampudi, T. Fukumizu et al., “Lipase localization in Rhizopus oryzae cells immobilized within biomass support particles for use as whole-cell biocatalysts in biodiesel-fuel production,” Journal of Bioscience and Bioengineering, vol. 101, no. 4, pp. 328–333, 2006. View at Publisher · View at Google Scholar · View at Scopus
  34. A. M. Klibanov, “Improving enzymes by using them in organic solvents,” Nature, vol. 409, no. 6817, pp. 241–246, 2001. View at Publisher · View at Google Scholar · View at Scopus
  35. A. Jayaprakash and P. Ebenezer, “Purification and characterization of Aspergillus japonicus lipase from a pig fat production medium,” Journal of Academia and Industrial Research, vol. 1, no. 1, pp. 1–7, 2012. View at Google Scholar
  36. D. Pokorny, A. Cimerman, and W. Steiner, “Aspergillus niger lipases: induction, isolation and characterization of two lipases from a MZKI A116 strain,” Journal of Molecular Catalysis B: Enzymatic, vol. 2, no. 4-5, pp. 215–222, 1997. View at Publisher · View at Google Scholar · View at Scopus
  37. N. C. Mhetras, K. B. Bastawde, and D. V. Gokhale, “Purification and characterization of acidic lipase from Aspergillus niger NCIM 1207,” Bioresource Technology, vol. 100, no. 3, pp. 1486–1490, 2009. View at Publisher · View at Google Scholar · View at Scopus
  38. M. de Fátima Silva Lopes, A. L. Leitão, M. Regalla, J. J. F. Marques, M. J. T. Carrondo, and M. T. B. Crespo, “Characterization of a highly thermostable extracellular lipase from Lactobacillus plantarum,” International Journal of Food Microbiology, vol. 76, no. 1-2, pp. 107–115, 2002. View at Publisher · View at Google Scholar · View at Scopus
  39. N. R. Kamini, J. G. S. Mala, and R. Puvanakrishnan, “Lipase production from Aspergillus niger by solid-state fermentation using gingelly oil cake,” Process Biochemistry, vol. 33, no. 5, pp. 505–511, 1998. View at Publisher · View at Google Scholar · View at Scopus
  40. A. Tsang, G. Butler, J. Powlowski, E. A. Panisko, and S. E. Baker, “Analytical and computational approaches to define the Aspergillus niger secretome,” Fungal Genetics and Biology : FG & B, vol. 46, pp. S153–S160, 2009. View at Publisher · View at Google Scholar · View at Scopus
  41. Z.-Y. Shu, J.-K. Yang, and Y.-J. Yan, “Purification and characterization of a lipase from Aspergillus niger F044,” Sheng Wu Gong Cheng Xue Bao, vol. 23, no. 1, pp. 96–101, 2007. View at Publisher · View at Google Scholar · View at Scopus
  42. S. M. Basheer, S. Chellappan, P. S. Beena, R. K. Sukumaran, K. K. Elyas, and M. Chandrasekaran, “Lipase from marine Aspergillus awamori BTMFW032: production, partial purification and application in oil effluent treatment,” New Biotechnology, vol. 28, no. 6, pp. 627–638, 2011. View at Publisher · View at Google Scholar · View at Scopus
  43. H. S. Hamdy and M. A. Abo-Tahon, “Extracellular lipase of Aspergillus terreus var. africanus (CBS 130.55): production, purification and characterisation,” Annals of Microbiology, vol. 62, no. 4, pp. 1723–1736, 2012. View at Publisher · View at Google Scholar · View at Scopus
  44. C. M. Romero, L. M. Pera, F. Loto, and M. D. Baigori, “Specific enzyme-catalyzed hydrolysis and synthesis in aqueous and organic medium using biocatalysts with lipase activity from Aspergillus niger MYA 135,” Catalysis Letters, vol. 142, no. 11, pp. 1361–1368, 2012. View at Publisher · View at Google Scholar · View at Scopus
  45. J. C. Mateos Diaz, J. A. Rodríguez, S. Roussos et al., “Lipase from the thermotolerant fungus Rhizopus homothallicus is more thermostable when produced using solid state fermentation than liquid fermentation procedures,” Enzyme and Microbial Technology, vol. 39, no. 5, pp. 1042–1050, 2006. View at Publisher · View at Google Scholar · View at Scopus
  46. M. A. F. Costa and R. M. Peralta, “Production of lipase by soil fungi and partial characterization of lipase from a selected strain (Penicillium wortmanii),” Journal of Basic Microbiology, vol. 39, no. 1, pp. 11–15, 1999. View at Publisher · View at Google Scholar
  47. A. Ibrik, H. Chahinian, N. Rugani, L. Sarda, and L.-C. Comeau, “Biochemical and structural characterization of triacylglycerol lipase from Penicillium cyclopium,” Lipids, vol. 33, no. 4, pp. 377–384, 1998. View at Publisher · View at Google Scholar · View at Scopus
  48. E. Rigo, J. L. Ninow, S. M. Tsai et al., “Preliminary characterization of novel extra-cellular lipase from Penicillium crustosum under solid-state fermentation and its potential application for triglycerides hydrolysis,” Food and Bioprocess Technology, vol. 5, no. 5, pp. 1592–1600, 2012. View at Publisher · View at Google Scholar · View at Scopus
  49. L. L. Zhao, J. H. Xu, J. Zhao, J. Pan, and Z. L. Wang, “Biochemical properties and potential applications of an organic solvent-tolerant lipase isolated from Serratia marcescens ECU1010,” Process Biochemistry, vol. 43, no. 6, pp. 626–633, 2008. View at Publisher · View at Google Scholar · View at Scopus
  50. P. Sarkar, S. Yamasaki, S. Basak, A. Bera, and P. K. Bag, “Purification and characterization of a new alkali-thermostable lipase from Staphylococcus aureus isolated from Arachis hypogaea rhizosphere,” Process Biochemistry, vol. 47, no. 5, pp. 858–866, 2012. View at Publisher · View at Google Scholar · View at Scopus
  51. J. Dong, P. Deng, and J. Xu, “Study of the effects of Cr ions on Yb in Cr,Yb:YAG crystal,” Optics Communications, vol. 170, no. 4–6, pp. 255–258, 1999. View at Publisher · View at Google Scholar · View at Scopus
  52. J. M. Palomo, G. Fernández-Lorente, J. M. Guisán, and R. Fernández-Lafuente, “Modulation of immobilized lipase enantioselectivity via chemical amination,” Advanced Synthesis and Catalysis, vol. 349, no. 7, pp. 1119–1127, 2007. View at Publisher · View at Google Scholar · View at Scopus
  53. G. Fernandez-Lorente, J. M. Palomo, Z. Cabrera, R. Fernandez-Lafuente, and J. M. Guisán, “Improved catalytic properties of immobilized lipases by the presence of very low concentrations of detergents in the reaction medium,” Biotechnology and Bioengineering, vol. 97, no. 2, pp. 242–250, 2007. View at Publisher · View at Google Scholar · View at Scopus
  54. B. S. Demir and S. S. Tükel, “Purification and characterization of lipase from Spirulina platensis,” Journal of Molecular Catalysis B: Enzymatic, vol. 64, no. 3-4, pp. 123–128, 2010. View at Publisher · View at Google Scholar · View at Scopus
  55. G. Fernández-Lorente, J. M. Palomo, M. Fuentes, C. Mateo, J. M. Guisán, and R. Fernández-Lafuente, “Self-assembly of Pseudomonas fluorescens lipase into bimolecular aggregates dramatically affects functional properties,” Biotechnology and Bioengineering, vol. 82, no. 2, pp. 232–237, 2003. View at Publisher · View at Google Scholar · View at Scopus
  56. C. Yang, F. Wang, D. Lan, C. Whiteley, B. Yang, and Y. Wang, “Effects of organic solvents on activity and conformation of recombinant Candida antarctica lipase A produced by Pichia pastoris,” Process Biochemistry, vol. 47, no. 3, pp. 533–537, 2012. View at Publisher · View at Google Scholar · View at Scopus
  57. E. H. Ahmed, T. Raghavendra, and D. Madamwar, “An alkaline lipase from organic solvent tolerant Acinetobacter sp. EH28: application for ethyl caprylate synthesis,” Bioresource Technology, vol. 101, no. 10, pp. 3628–3634, 2010. View at Publisher · View at Google Scholar · View at Scopus
  58. L. Daoud, J. Kamoun, M. B. Ali et al., “Purification and biochemical characterization of a halotolerant Staphylococcus sp. extracellular lipase,” International Journal of Biological Macromolecules, vol. 57, pp. 232–237, 2013. View at Publisher · View at Google Scholar · View at Scopus
  59. D. S. Dheeman, S. Antony-Babu, J. M. Frías, and G. T. M. Henehan, “Purification and characterization of an extracellular lipase from a novel strain Penicillium sp. DS-39 (DSM 23773),” Journal of Molecular Catalysis B: Enzymatic, vol. 72, no. 3-4, pp. 256–262, 2011. View at Publisher · View at Google Scholar · View at Scopus
  60. X. Li, P. Qian, S.-G. Wu, and H.-Y. Yu, “Characterization of an organic solvent-tolerant lipase from Idiomarina sp. W33 and its application for biodiesel production using Jatropha oil,” Extremophiles, vol. 18, no. 1, pp. 171–178, 2014. View at Publisher · View at Google Scholar · View at Scopus
  61. J.-L. Xia, B. Huang, Z.-Y. Nie, and W. Wang, “Production and characterization of alkaline extracellular lipase from newly isolated strain Aspergillus awamori HB-03,” Journal of Central South University of Technology, vol. 18, no. 5, pp. 1425–1433, 2011. View at Publisher · View at Google Scholar · View at Scopus
  62. I. Mayordomo, F. Randez-Gil, and J. A. Prieto, “Isolation, purification, and characterization of a cold-active lipase from Aspergillus nidulans,” Journal of Agricultural and Food Chemistry, vol. 48, no. 1, pp. 105–109, 2000. View at Publisher · View at Google Scholar · View at Scopus
  63. N. R. Kamini, T. Fujii, T. Kurosu, and H. Iefuji, “Production, purification and characterization of an extracellular lipase from the yeast, Cryptococcus sp. S-2,” Process Biochemistry, vol. 36, no. 4, pp. 317–324, 2000. View at Publisher · View at Google Scholar · View at Scopus
  64. K. Yamamoto and N. Fujiwara, “The hydrolysis of castor oil using a lipase from Pseudomonas sp. f-B-24: positional and substrate specificity of the enzyme and optimum reaction conditions,” Bioscience, Biotechnology and Biochemistry, vol. 59, no. 7, pp. 1262–1266, 1995. View at Publisher · View at Google Scholar · View at Scopus