Conditioned Media from Human Adipose Tissue-Derived Mesenchymal Stem Cells and Umbilical Cord-Derived Mesenchymal Stem Cells Efficiently Induced the Apoptosis and Differentiation in Human Glioma Cell Lines In Vitro

Figure 8

Matrigel invasion assay. U251 cells or U87 cells (induced or noninduced) were placed in transwells with 8 μm pore size polycarbonate filters, precoated with Matrigel. The lower wells were filled with DMEM with 10% fetal bovine serum. The cells were incubated at 37°C for 24 hours. Nonmigratory cells on the upper surface of the transwells were removed and the migratory cells from the lower surface of the transwell were fixed and stained with 1% crystal violet. Cells were photographed and average cells per field were calculated. Experiments were done in triplicates on three times independently.