Altered Pulmonary Lymphatic Development in Infants with Chronic Lung Disease

Figure 1

Lung specimens from a 31-week control infant ((a)–(c1)) and 31-week infant with moderate CLD ((d)–(f1)). Bronchovascular bundle ((a), (d) and (a1), (d1)), respiratory bronchiolar ((b), (e) and (b1), (e1)), and alveolar ductal ((c), (f) and (c1), (f1)) levels are displayed. ((a1), (b1), (c1)) and ((d1), (e1),(f1)) are higher magnifications (10x, 20x, 40x, resp.) of regions outlined in ((a), (b), (c)) and ((d), (e), (f)), respectively (magnification 4x). Slides are double immunostained with anti-human podoplanin and anti-human CD31 antibodies. Podoplanin and CD31 stains are selective for lymphatic and blood vasculature, respectively. Lymphatic vessels are stained brown (arrows) and are associated with arteries and arterioles. Arteries and arterioles are stained purple (arrowheads).