Cardioprotective Effects of Osteopontin-1 during Development of Murine Ischemic Cardiomyopathy
Figure 1
Osteopontin-deficiency leads to cardiomyocyte loss and poor left ventricular function. (a) Osteopontin mRNA-expression in whole WT-mouse hearts during repetitive I/R and (b) in Langendorff-isolated adult WT-cardiomyocytes after 3 days I/R compared to sham. (c) Representative HE-stained section of WT-heart after 7 days I/R shows interstitial cellular infiltration in contrast to (d) irreversible loss of cardiomyocytes in OPN−/−-mice (arrow). (e) Fractional shortening was impaired in both strains, but loss of left ventricular contractility was significantly reduced in OPN−/−-mice after 7 d I/R compared to WT. (f) Anterior wall thickening was significantly reduced in OPN−/−-mice after 7 days I/R. /group. Scale bars represent 50 m. RT-qPCR using Taqman and mRNA-expression is related to controls and GAPDH using comparative Ct-method. Bracket indicates between genotypes; indicates versus respective shams.
