Cardioprotective Effects of Osteopontin-1 during Development of Murine Ischemic Cardiomyopathy

Figure 6

Attenuated remodeling in ischemic OPN⁻/⁻-hearts. (a) Representative section of left ventricle after 7 days I/R in a WT-heart shows numerous 𝛼-smac positive myofibroblasts (black arrows) in the ischemic myocardium. (b) At the same time point, 𝛼-smac positive signals (black arrow) were almost absent in small, nontransmural infarctions (white arrows) of OPN⁻/⁻-hearts. mRNA-induction of (c) tenascin-C (TNC), (d) MMP-2, (e) MMP-9, (f) MMP-12, (g) MMP-13, (h) TIMP-1, and (i) TIMP-2 in WT- and OPN⁻/⁻-hearts during repetitive I/R. 𝑛=8--11/group. Scale bars represent 50 𝜇m. RT-qPCR using Taqman and mRNA-expression is related to controls and GAPDH using comparative ΔΔCt-method. Bracket indicates 𝑃≤0.05 between genotypes; ∗ indicates 𝑃≤0.05 versus respective shams.

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