Cardioprotective Effects of Osteopontin-1 during Development of Murine Ischemic Cardiomyopathy

Figure 7

Expression of remodeling markers in myofibroblasts in vitro. Cultured myofibroblasts from WT- and OPN⁻/⁻-hearts were stimulated with human TGF-𝛽1 or PBS as control under normoxia (21% O₂) or hypoxia (2% O₂). mRNA-expression of (a) OPN-1 in WT-cells only. mRNA-expression of (b) galectin-3, (c) CCL2, (d) tenascin-C (TNC), (e) MMP-9, (f) MMP-12, and (g) TIMP-1. 𝑛=5--7/group. RT-qPCR using Taqman and mRNA-expression is related to controls and 18 S (in vitro) using comparative ΔΔCt-method. Bracket indicates 𝑃≤0.05 between genotypes; ∗ indicates 𝑃≤0.05 versus 6 h + PBS normoxia group (control).

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