BioMed Research International / 2014 / Article / Fig 1

Research Article

Entamoeba histolytica and E. dispar Calreticulin: Inhibition of Classical Complement Pathway and Differences in the Level of Expression in Amoebic Liver Abscess

Figure 1

EhCRT/EdCRT interaction with human C1q (ELISA). Microtiter wells were coated with 2.5 to 200  M of the EhCRT or EdCRT and incubated with 1 : 10 diluted normal human serum supplemented with 4  g of human C1q; the interaction of EhCRT-C1q was revealed using an anti-human C1q monoclonal antibody produced in mice and then an anti-mouse IgG produced in goat conjugated to peroxidase. Values are the mean of three different assays performed in triplicate ± SD.