BioMed Research International / 2014 / Article / Fig 2

Research Article

Entamoeba histolytica and E. dispar Calreticulin: Inhibition of Classical Complement Pathway and Differences in the Level of Expression in Amoebic Liver Abscess

Figure 2

EhCRT inhibits classical pathway-mediated hemolysis. Different proteins of (CRT+) EhCRT or EdCRT; (CRT−) BSA or EhCRT-C was added to 1 : 10 dilution of NHS (as source of C1q), incubated 30 min at 37°C, and then added to 108 cell/mL of EA; the mixtures were incubated for 60 min at 37° C. After centrifugation, the OD (550 nm) of the supernatants was measured. The percentage of lyses was calculated using as reference the 100% lyses of erythrocytes in water. Values are the mean of three independent experiments ± SD. Differences between groups * were compared through ANOVA test detecting statistical significance ( ).