Entamoeba histolytica and E. dispar Calreticulin: Inhibition of Classical Complement Pathway and Differences in the Level of Expression in Amoebic Liver Abscess

Figure 3

EhCRT-human C1q on the activation of classical complement pathway: hemolysis assay. 1: corresponding to NHS (positive control); 2: human C1q-depleted serum (NHSC1q⁻); 3: (NHSC1q⁻) + C1q; 4: (NHSC1q⁻) + C1q + EhCRT; 5: (NHSC1q⁻) + C1q + EhCRT + IgG anti-EhCRT. Assays were performed in triplicate; values are the mean of three different experiments ± SD. Differences between groups *, ●, and ▼ were compared through ANOVA test. Statistical significance (𝑃=0.012).