Entamoeba histolytica and E. dispar Calreticulin: Inhibition of Classical Complement Pathway and Differences in the Level of Expression in Amoebic Liver Abscess
Amoebicidal activity of human serum. Trophozoites of E. histolytica and E. dispar were harvested by ice bath and then centrifuged at 500 g for 10 min, washed, counted, adjusted to a cell density of , and incubated with TYIS-33 medium added with 40% NHS at different times; viability was assessed by trypan blue exclusion technique. To estimate the inhibition of lyses due to interaction of EhCRT-C1q, 10 g of rEhCRT was added to NHS incubating during 10 min, and the mixture was then added to trophozoites suspension. The percent of lyses was defined as the decrease of trophozoites viability in the presence of NHS compared with the heat-inactivated human serum control. Differences between groups +, ●, and ▼ were compared through Student’s -test detecting statistical significance ().