Adipose Tissue-Derived Stem Cell Secreted IGF-1 Protects Myoblasts from the Negative Effect of Myostatin
Relative quantification of MyoD mRNA is shown as mean ± SD percentage of MyoD mRNA expression in murine myoblasts after 72 h myostatin treatment. Myostatin exposure significantly reduced MyoD mRNA to 22.8% (±4.6%, ) in comparison to untreated myoblasts. Due to the effect of conditioned medium from wild type ASCs MyoD, mRNA significantly increased to 51.2% (±5.9%, ) when compared to myostatin treatment. But this effect was accountable for IGF-1 by 87.3% since IGF-1 depleted CM decreased MyoD mRNA level to 26.4% (±3.4%, n.s. compared to myostatin treatment) (, ).