Adipose Tissue-Derived  Stem Cell Secreted IGF-1 Protects Myoblasts from the Negative Effect of Myostatin

<table>Relative quantification of MyoD mRNA is shown as mean ± SD percentage of MyoD mRNA expression in murine myoblasts after 72 h myostatin treatment. Myostatin exposure significantly reduced MyoD mRNA to 22.8% (±4.6%, <svg height="13.95" id="M57" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 81.462502 13.95" width="81.462502" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>) in comparison to untreated myoblasts. Due to the effect of conditioned medium from wild type ASCs MyoD, mRNA significantly increased to 51.2% (±5.9%, <svg height="13.95" id="M58" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 81.462502 13.95" width="81.462502" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>) when compared to myostatin treatment. But this effect was accountable for IGF-1 by 87.3% since IGF-1 depleted CM decreased MyoD mRNA level to 26.4% (±3.4%, n.s. compared to myostatin treatment) (<svg height="11.375" id="M59" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 42.5625 11.375" width="42.5625" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>, <svg height="11.125" id="M60" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 36.237499 11.125" width="36.237499" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg>).</table>

BioMed Research International

fig6

Figure 6

Figure 6: Adipose Tissue-Derived  Stem Cell Secreted IGF-1 Protects Myoblasts from the Negative Effect of Myostatin 