Research Article
Adipose Tissue-Derived Stem Cell Secreted IGF-1 Protects Myoblasts from the Negative Effect of Myostatin
Table 1
Primer sets for SYBR Green assay.
| | Primer | Tm | | Sequence |
| | Actin |
55°C | Sense | CAGGTCCAGACGCAGGATGGC | | Antisense | CTACAATGAGCTGCGTGTGGC |
| | MyoD | 57°C | Sense | GGTCTGGGTTCCCTGTTCTGTGT | | Antisense | CCCCGGCGGCAGAATGGCTACG |
| | GAPDH | 67°C | Sense | AGCCACATCGCTCAGACACC | | Antisense | GTACTCAGCCGCCAGCATCG |
| | IGF-1 | 60°C | Sense | GCTGGTGGAAGCTCTTCAGTTC | | Antisense | AGCTGACTTGGCAGGCTTGAG |
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