Mesenchymal Stem Cells Expressing Brain-Derived Neurotrophic Factor Enhance Endogenous Neurogenesis in an Ischemic Stroke Model
MSCs-BDNF increased endogenous neurogenesis in the ischemic stroke model. (a) Area of the SVZ exhibiting migrating neuroblasts (DCX; green). Coexpression of BrdU (red) and DCX (green) in cells of the SVZ. Arrows indicate double-positive (BrdU/DCX) cells. MSCs-BDNF led to an increase in the number of new neuronal cells after cell transplantation (scale bar: (A)–(I); 20 μm, (J)–(L); 10 μm). (b) Significant increase in the number of BrdU+ cells in the SVZ of the MSCs and MSCs-BDNF groups compared with the PBS group (*). (c) Quantification of DCX immunoreactivity (* and **). (d) Quantification of DCX/BrdU double-positive cells in the SVZ (* and **). (e) Coexpression of BrdU (red) and NeuN (mature neuronal marker; green) in the ipsilateral striatum at 4 weeks after MCAO (scale bar: 10 μm). (f) Quantification of NeuN/BrdU double-positive immunoreactivity (* and **).