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BioMed Research International
Volume 2014 (2014), Article ID 132349, 11 pages
Research Article

Expressions of ABCG2, CD133, and Podoplanin in Salivary Adenoid Cystic Carcinoma

1Medical College of Stomatology, Dalian Medical University, 9 Western Section, Lvshun South Street, Dalian 116044, China
2Department of Histology, Nihon University School of Dentistry at Matsudo, 2-870-1 Sakaemachi-nishi, Matsudo 271-8587, Japan
3Comprehensive Transplant Center, Department of Surgery, Feinberg School of Medicine, Northwestern University, 300 E. Superior Street, Chicago, IL 60611, USA
4Department of Health Statistics, Dalian Medical University, 9 Western Section, Lvshun South Street, Dalian 116044, China
5Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Okayama 700-8525, Japan

Received 11 December 2013; Accepted 13 March 2014; Published 6 April 2014

Academic Editor: Jun Liao

Copyright © 2014 Wuwei Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Adenoid cystic carcinoma (ACC) is one of the most common salivary gland malignant tumors with a high risk of recurrence and metastasis. Current studies on cancer stem cells (CSCs) have verified that CSCs are the driving force behind tumor initiation and progression, suggesting that new cancer therapies may be established by effectively targeting and killing the CSCs. The primary goal of this study is to investigate the expression patterns of ABCG2, CD133, and podoplanin in ACC of minor salivary glands by immunohistochemistry analysis. We found that ABCG2 was weakly expressed in normal looking salivary gland tissues. A significant upregulation of ABCG2 expression in ACC was observed with a similar expression pattern of Ki-67. CD133 was detected in apical membrane of epithelial cells and podoplanin was expressed positively in myoepithelial cells of both normal looking tissue and ACC. However, no significant difference was found of the expression pattern of CD133 and podoplanin between normal looking tissues and ACC. Our observations suggest that CSCs may exist in quiescent cells with ABCG2 positive staining, which are surrounded by cells with positive expression of ABCG2 and Ki-67 in ACC, and costaining with ABCG2 and Ki-67 may help predict the location of CSCs.