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BioMed Research International
Volume 2014, Article ID 134236, 8 pages
Research Article

Dynamic of the Cellular Immune Response at the Dermal Site of Leishmania (L.) amazonensis and Leishmania (V.) braziliensis Infection in Sapajus apella Primate

1Infectious Diseases Laboratory (LIM-50), Pathology Department, Medical School of São Paulo University, Avenida Dr. Arnaldo, 455-1° Andar, Sala 1209, 01246-903 São Paulo, SP, Brazil
2Leishmaniasis Laboratory, Evandro Chagas Institute, Ministry of Health, Rodovia BR 316 s/n, 67030-000 Ananindeua, PA, Brazil
3Tropical Medicine Nucleus, Pará Federal University, Avenida Generalíssimo Deodoro 92, 66055-240 Belém, PA, Brazil

Received 10 February 2014; Revised 21 June 2014; Accepted 13 August 2014; Published 28 August 2014

Academic Editor: Amogh A. Sahasrabuddhe

Copyright © 2014 Márcia Dalastra Laurenti et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The purpose of this study was to characterize the immunopathological response in the skin of S. apella infected with Leishmania (L.) amazonensis and L. (V.) braziliensis parasites, the main causative agents of localized cutaneous leishmaniasis in South America. In infected animals, amastigote forms of L. (L.) amazonensis could be detected till 120 days postinfection (PI), while, in L. (V.) braziliensis infection, parasites could be detected until 180 days PI in the skin sections. CD20+ cells were detected throughout the experimental time in both groups as well as in CD3+ cells, which appeared to be activated because high densities of inducible nitric oxide synthase (iNOS+) cells were detected at 60 and 90 days PI in both studied groups. After 60 and 120 days PI, decrease in iNOS+ cells was observed in L. (L.) amazonensis and L. (V.) braziliensis, respectively, which was associated with parasite clearance. Increase in lysozyme+ cells was observed during the experimental infections, which also can be associated with parasite killing.