Negative Regulation of GADD34 on Myofibroblasts during Cutaneous Wound Healing
Figure 4
GADD34 suppresses α-SMA expression by TGF β signal pathway. (a) Expressions of mRNA of dermal samples from WT and GADD34−/− mice were determined via real-time PCR. Expression levels are expressed relative to β-actin utilizing the ddCt method. Statistics represent 3 independent experiments and results shown are mean ± SD. R-Smad signaling was analyzed by western blot analysis. Cell lysates were prepared from wound site tissues at indicated time point after wounding. Bands were detected with anti-phospho-Smad2/3 antibodies (b). Statistics represent 3 independent experiments.