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(a) Cluster dendrogram
138350.fig.004b
(b) Cluster dendrogram: High variance
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(c) Cluster dendrogram: F-Test (best P-value)
Figure 4: Genome-wide profiling of spermatogonia in comparison with htFibs and hESCs. (a) Dendrogram based on the whole transcriptome, calculated using complete linkage clustering and Euclidean distance. Samples were grouped almost perfectly according to their cell type. Short-term cultured spermatogonia (spermatogonia_short, left hand side of the tree) were poorly related to other cell types. hESCs have a rather high degree of relationship to fibroblasts. (b) Dendrogram based on the 150 features with highest variances, calculated using complete linkage clustering and Euclidean distance. All cell types were grouped correctly into subtrees. (c) Dendrogram based on the 150 features with highest discrimination power assessed by the value of an F-test. Using a set of markers designed for distinguishing the groups, separation of the cell types was even stronger in comparison with (a) and (b). The overall topology remains very similar in all cases.